Características do Software Gen5 para Detecção
Software
Arquivos de Exemplos
Absorbance
- dsDNA direct quantification at 260 nm and purity assessment using the 260 / 280 ratio. Well-to-well pathlength correction is used to convert measurement to a 1 cm pathlength equivalent (07-Jun-11)
- BCA protein assay with protein concentration curve (standard curve) and calculation of unknown concentrations (08-Jun-11)
- Using a calibration curve protocol when the standard or calibrator wells are on the first plate and the sample are on the next plates (08-Jun-11)
- End point ELISA assay with quantitative analysis (calculation of unknown concentrations) and qualitative analysis (“positive”, “negative”, “re-retest” calls) (08-Jun-11)
- Beta galactosidase kinetic assay with calculation of mean slope and dose-response curve (08-Jun-11)
- Michaelis Menten Analysis of Kinetic Data (08-Jun-11)
- Paneling multiple assays on one plate using Gen5’s “panel” functionality (08-Jun-11)
- Monitoring the growth of various yeast strains using light scattering at 600 nm (08-Jun-11)
- AlamarBlue A (15-Nov-12)
- BioCell (15-Nov-12)
- Bradford Protein Assay (20-Nov-12)
- Direct Oligo Quantification Blank Plate (20-Nov-12)
- Direct RNA Quantification (20-Nov-12)
- DsDNA Quantification Blank Plate (20-Nov-12)
- EDTA Inhibition Assay (20-Nov-12)
- IC50 (Toxicity) Assay (20-Nov-12)
- Kinetic Endotoxin Chromogenic (20-Nov-12)
- Lactate - NADH (20-Nov-12)
- Lowry Protein Assay (21-Nov-12)
- Particulate Test A320 (21-Nov-12)
- Phenol Test (21-Nov-12)
- Platelet aggregation assay using light scattering at 630 nm with calculation of % transmittance to determine cut-offs (04-Jan-13)
- Monitoring bacterial cell growth in a 384-well plate using light scattering at 600 nm (04-Jan-13)
- Monitoring a chromosomal luciferase-gene reporter at different stages of S. aureus growth (04-Jan-13)
- 120817 SoluLink biotin labeling Take3 (12-Feb-13)
- Quantification of Cy3 labeling efficiency for dsDNA (18-Jun-14)
- Quantification of Cy5 labeling efficiency for dsDNA (18-Jun-14)
- Quantification of Cy3 labeling efficiency for RNA (18-Jun-14)
- Quantification of Cy5 labeling efficiency for RNA (18-Jun-14)
- Quantification of Cy3 labeling efficiency for ssDNA (18-Jun-14)
- Quantification of Cy5 labeling efficiency for ssDNA (18-Jun-14)
- Estimating Relative Potency for CVB using Gen5™ Software (16-Jul-14)
- Precice Freshness Assay (08-Aug-14)
- 150324 LoBase plate UV-Neo (18-Dec-15)
- Qualitative Clinical Assay (20-Sep-17)
- Quantitative Clinical Assay (20-Sep-17)
- ADH kinetic assay (22-Sep-17)
AlphaScreen / AlphaLISA / SureFire
- Insulin titration using AlphaLISA assay kit (07-Jun-11)
- cAMP titration using a cAMP AlphaScreen assay kit (07-Jun-11)
- Rapamycin dose-response curve and EC50 calculation using a Surefire assay kit (07-Jun-11)
- AlphaLISA Drug Tolerance (05-Jun-13)
- Cy5 Protein A Antibody Screen w-std. curve (14-Jul-15)
- SureFire AlphaLISA p-ERK lysate std curve (14-Jul-15)
Fluorescence - Time Resolved / TR-FRET
- HTRF® TR-FRET reader control kit data and data analysis steps (08-Jun-11)
- LanthaScreen TR-FRET assay reading parameters and dilution curve (08-Jun-11)
- LANCE TR-FRET reading parameters and cAMP test kit results (03-Oct-11)
- LANCE TR-FRET cAMP (27-Nov-12)
- TRF Cyclic AMP Delfia with data filters (29-Nov-12)
- Cisbio HTRF assay reading parameters, dose-response curves and Z’-factor calculation (08-Jan-13)
- BRD4 + -JQ1 dose response (12-Feb-13)
- DELFIA ADCC Cytotoxicity Assay reading parameters and % Maximum Cytotoxicity calculations for EC50 value (08-Mar-13)
- EPIgeneous G9a Titration (23-Dec-14)
- AgilentpHX (01-May-18)
- AgilentXO2 (01-May-18)
Fluorescence Intensity
- High-definition area scan (99 x 99 points) of adherent cell layer labeled with calcein (06-Jun-11)
- Area scan (31 x 31 points) on full 24-well microplate with adherent cell layer labeled with calcein (06-Jun-11)
- 3D excitation/emission fluorescent spectral scan plotted in Excel using PowerExport (07-Jun-11)
- Kinetic measurement of an Omnia kinase assay using both filters and monochomators on a Hybrid microplate reader, allows comparing filter and monochromator data (08-Jun-11)
- Synergy injection system used to study protein folding by measuring tryptophan UV fluorescence (08-Jun-11)
- GeneBLAzer FRET assay reading parameters, EC50 and Z prime calculation (08-Jun-11)
- Using a multi-plate protocol when data analysis needs to occur across multiple plates (08-Jun-11)
- Creating a protocol and plate layout using multiple standard curves on the same plate (08-Jun-11)
- Reading parameters and data analysis for an ORAC (Oxygen Radical Absorbance Capacity) assay using a Synergy with injectors (08-Jun-11)
- PicoGreen dsDNA fluorescence assay with standard curve and calculation of unknown sample concentrations. (08-Jun-11)
- Spectral scanning of multiple compounds with various excitation and emission peaks and overlaying them on one graph (08-Jun-11)
- High-definition area scan (99 x 99 points) on a well of a 96-well microplate (03-Oct-11)
- SNP genotyping FRET assay in a 1536 well microplate using KBioscience’s KASP assay (03-Oct-11)
- Alamar Blue F (21-Nov-12)
- Alexa Fluor 488 (21-Nov-12)
- AttoPhos (21-Nov-12)
- Calcium Green (21-Nov-12)
- Caspase-3 AMC Substrate (21-Nov-12)
- Caspase-3 Assay (Assay Designs) (21-Nov-12)
- Caspase-6 Assay (Assay Designs) (21-Nov-12)
- Catalase (Amplex Red) (26-Nov-12)
- CBQCA Protein Assay (26-Nov-12)
- Cholesterol (Amplex Red) (27-Nov-12)
- CyQuant Cell Proliferation Assay (27-Nov-12)
- DAPI (27-Nov-12)
- EGFP (27-Nov-12)
- EnzChek Amylase Assay (27-Nov-12)
- Ethidium Bromide (27-Nov-12)
- FAM (27-Nov-12)
- Fluorescamine Protein Quantitation (27-Nov-12)
- Glucose (Amplex Red) (27-Nov-12)
- Hoechst 33258 (High Concentrations) (27-Nov-12)
- Hoechst 33258 (Low Concentrations) (27-Nov-12)
- Hydrogen Peroxide (Amplex Red) (27-Nov-12)
- OliGreen High Concentrations (27-Nov-12)
- OliGreen Low Concentrations (27-Nov-12)
- OPA Protein Quantitation (27-Nov-12)
- Propidium Iodide (27-Nov-12)
- Quanta Blu Assay (27-Nov-12)
- Resorufin (27-Nov-12)
- RiboGreen Low Concentrations (27-Nov-12)
- RiboGreen High Concentrations (27-Nov-12)
- ROX (27-Nov-12)
- TAM (27-Nov-12)
- IL6 Demo assay on Optimiser (14-Mar-13)
- H4 Standard Curve (14-Aug-14)
- 150324 LoBase plate FL-Neo (18-Dec-15)
Fluorescence Polarization
- Parallel Line Analysis applied to fluorescence polarization data from a Predictor hERG assay (07-Jun-11)
- Polarization with Fluorescein Label (27-Nov-12)
- Set7-9 Titrations (12-Feb-13)
- Critical micelle concentration determination using fluorescence polarization (08-Mar-13)
- Dose Response as a Function of Detection Measurements per Well (13-Mar-13)
- UV polarization (13-Oct-14)
Luminescence
- Dual luciferase assay system (DLR) assay automated on a Synergy reader using the system’s injectors, and calculation of the DLR ratio (08-Jun-11)
- Luminescence-based assay using Promega’s GloSensor technology, with dose-response curves and EC50 calculations (08-Jun-11)
- ATP Flash (27-Nov-12)
- ATP Glow (27-Nov-12)
- dsDNA Quantitation (27-Nov-12)
- Dual Luciferase Glow (Dual Glo) (27-Nov-12)
- Fast Kinetic Luminescence (29-Nov-12)
- Long Kinetic Luminescence (29-Nov-12)
- Luciferase Flash (29-Nov-12)
- Luciferase Glow (29-Nov-12)
- MycoAlert (29-Nov-12)
- Qualitative ELISA Glow Luminescence (29-Nov-12)
- Quantitative ELISA Glow Luminescence (29-Nov-12)
- Monitoring a chromosomal luciferase-gene reporter at different stages of S. aureus growth (04-Jan-13)
- Promega ADCC reporter bioassay reading parameters and fold induction calculations for EC50 value (12-Feb-13)
- DiscoveRx InCELL Hunter Epigenetics Assay reading parameters and fold induction calculations for Z’ and EC50 value (12-Feb-13)
- HDAC-Glo I/II Assay Reading Parameters and Data Analysis for Assay Optimization and Screening (27-Mar-13)
- Solution ELISA Prozone and Sensitivity assay (05-Jun-13)
Sample Experiments with Data
System Files - Plate Types
- BioTek Counting Chamber (11-Jun-15)
- BioTek Adapter for Trevigen 96-well Comet Chip (01-Dec-15)
- iNCYTO C-chip (29-Oct-15)
- Ibidi µ-Slide VI (07-Jan-16)
- Ibidi µ-Slide | 0.6 Luer (07-Jan-16)
- Ibidi µ-Slide y-shaped (07-Jan-16)
- InSphero GravityTRAP assay plate (07-Jan-16)
- 1450541 Multi-vessel Adapter (25-Feb-16)
- Costar 3506 6-well plate (07-Mar-16)
- Costar 3512 12-well plate (07-Mar-16)
- Costar 3524 24-well plate (07-Mar-16)
- Costar 3548 48-well plate (07-Mar-16)
- MilliporeStageInsert (27-Jun-16)
- ONIXB04A (12-May-16)
- ONIXC04A (12-May-16)
- ONIXM04G (12-May-16)
- ONIXM04L (12-May-16)
- ONIXM04S (12-May-16)
- ONIXY04C (12-May-16)
- ONIXY04D (12-May-16)
- Greiner SensoPlate Plus, 384-well (13-May-16)
- T75 flask stage insert (27-Jul-16)
- T25 Flask Adapter for Corning PN 353108 (02-Mar-17)
- 4-position slide holder (28-Aug-17)
System Files - Synergy Neo2 Filter Cubes
- Cube 1 PN 1035001 (20-Nov-12)
- Cube 2 PN 1035002 (20-Nov-12)
- Cube 3 PN 1035003 (20-Nov-12)
- Cube 4 PN 1035004 (20-Nov-12)
- Cube 5 PN 1035005 (20-Nov-12)
- Cube 11 PN 1035011 (20-Nov-12)
- Cube 12 PN 1035012 (20-Nov-12)
- Cube 13 PN 1035013 (20-Nov-12)
- Cube 14 PN 1035014 (20-Nov-12)
- Cube 15 PN 1035015 (20-Nov-12)
- Cube 16 PN 1035016 (20-Nov-12)
- Cube 17 PN 1035017 (20-Nov-12)
- Cube 41 PN 1035041 (20-Nov-12)
- Cube 42 PN 1035042 (20-Nov-12)
- Cube 43 PN 1035043 (20-Nov-12)
- Cube 44 PN 1035044 (20-Nov-12)
- Cube 45 PN 1035045 (20-Nov-12)
- Cube 46 PN 1035046 (20-Nov-12)
- Cube 47 PN 1035047 (20-Nov-12)
- Cube 48 PN 1035048 (20-Nov-12)
- Cube 49 PN 1035049 (20-Nov-12)
- Cube 61 PN 1035061 (20-Nov-12)
- Cube 62 PN 1035062 (20-Nov-12)
- Cube 63_PN 1035063 (20-Nov-12)
- Cube 64_PN 1035064 (20-Nov-12)
- Cube 65 PN 1035065 (20-Nov-12)
- Cube 66 PN 1035066 (20-Nov-12)
- Cube 67 PN 1035067 (20-Nov-12)
- Cube 101 PN 1035101 (20-Nov-12)
- Cube 102 PN 1035102 (20-Nov-12)
- Cube 103 PN 1035103 (20-Nov-12)
- Cube 104 PN 1035104 (20-Nov-12)
- Cube 105 PN 1035105 (20-Nov-12)
- Cube 106 PN 1035106 (20-Nov-12)
- Cube 107 PN 1035107 (20-Nov-12)
- Cube 108 PN 1035108 (20-Nov-12)
- Cube 109 PN 1035109 (20-Nov-12)
- Cube 110 PN 1035110 (20-Nov-12)
- Cube 111 PN 1035111 (20-Nov-12)
- Cube 112 PN 1035112 (20-Nov-12)
- Cube 113 PN 1035113 (20-Nov-12)
- Cube 114 PN 1035114 (20-Nov-12)
- Cube 115 PN 1035115 (05-Jun-13)
- Cube 116 PN 1035116 (05-Jun-13)
- Cube 117 PN 1035117 (20-Feb-14)
- Cube 71 PN 1035071 (06-Jan-15)
- Cube 72 PN 1035072 (06-Jan-15)
- Cube 118 PN 1035118 (01-Feb-16)
- Cube 122 PN 1035122 (09-Feb-16)
- Cube 74 PN 1035074 (09-Feb-16)
- pH-Xtra Glycolysis (27-Jun-18)