Microscópio Automático Lionheart FX
O Microscópio Automático Lionheart™ FX é um sistema compacto e completo de microscopia, para uma ampla variedade de aplicações em captura de imagens. Conta com objetivas de aumentos até 60x em ar; 60x e 100x de imersão em óleo, com canais de fluorescência, campo claro, campo claro em cores e contraste de fase, para uma grande variedade de aplicações. Uma tampa opcional de controle ambiental permite incubação até 40°C e controle de níveis de CO2/O2, e a câmara de umidade otimiza condições para ensaios de longa duração de captura de imagens com células vivas, e o módulo opcional de injetores duplos de reagentes facilita ensaios de cinéticas rápidas. O Lionheart FX, em conjunto com o software Gen5, compreendem o conceito Augmented Microscopy™ - a automação de captura de imagens, processamento, análise e desenvolvimento de dados e imagens prontos para publicações.
- Microscopia digital automática até 60x em ar; 60x e 100x de imersão em óleo
- Captura de imagens por fluorescência, campo claro, campo claro em cores e contraste de fase
- Contagem de células vivas sem marcação ("label-free") para estudos de proliferação celular
- Permite ensaios com células vivas, graças a controle de condições ambientais
- Mais de 20 opções de cubos de LEDs/filtros para imagens em muitas aplicações
- Design compacto, com rápida instalação e configuração
O que há de Novo
The Scratch Assay Starter Kit automates cell migration and wound healing assays, used along with BioTek’s Lionheart FX Automated Microscope or Cytation 5 Cell Imaging Multi-Mode Reader.
Special Imaging Offer! Purchase a Lionheart or Cytation and receive patented Laser Autofocus Free!
aplicações
Lionheart FX is designed for easy implementation of a broad range of fixed and live cell assay workflows. BioTek’s concept of Augmented Microscopy™ brings automated image capture, processing, analysis and publication-ready images and data to the benchtop – for many applications, including these important areas:
- Cell migration and invasion assays
- Live cell imaging
- High resolution microscopy
- Immunofluorescence
- Phenotypic assays
- Histology
The Scratch Assay Starter Kit automates cell migration and wound healing assays:
AutoScratch automatically makes consistent, repeatable scratch wounds.
The Scratch Assay App automatically calculates % confluence, wound width and maximum healing rate (max V).
AutoScratch creates scratch wounds with excellent performance, compared to manual methods.
Adding 10 µM ATP activates HeLa endogenously expressed P2Y receptor
Rat kidney mitosis, prophase at 60x.
Visit our Applications pages to learn more about all of the critical applications enabled with Lionheart FX.
BioTek Field Applications Scientists (FAS) and Product Specialists provide unparalleled scientific support, assisting with experimental planning and assay optimization on BioTek’s instrumentation and software. Learn how they can help move your science forward… faster!
Características
Automated digital microscopy up to 100x
Lionheart FX with Augmented Microscopy automates make your imaging workflow to yield amazing images and powerful data. Automated image capture starts with image-based and laser autofocus, plus auto LED and exposure. Automated image pre-processing optimizes images for downstream analysis, from cell counting to characterization of subcellular details.
Four imaging modes, multiple imaging processes
With brightfield, color brightfield, phase contrast and fluorescence imaging in four channels – and nearly twenty available filter/LED cubes, Lionheart FX offers fantastic versatility that isn’t limited to image capture. Follow your imaging processes through z-stacking to z-projection, montage collection to stitching and digital phase contrast to analysis, all one hardware and software platform.
Label-free cell counting
It’s fast and simple to count live cells using the available high contrast brightfield option. Higher contrast allows a direct and label-free method for object identification using Gen5 software to count cells and characterize cell proliferation in kinetic studies.
Kinetic live cell assay support
Several options are available to support live cell assays with Lionheart FX, including an environmental cover to maintain temperature and gas levels within the system. Long-term live cell kinetics are supported, using the unique humidity chamber that fits on the stage itself, keeping the live cell in a completely nurturing environment. A dual reagent injector provides quick inject/image capability for observing fast reactions.
More than 20 imaging filter/LED cubes
BioTek has the most comprehensive selection of imaging filter and LED cubes available for use with our Lionheart and Cytation imagers. This large selection of colors enables image capture for a broad range of applications. Download our convenient Imaging Cube Selection Guide here.
Quick installation and easy setup
Fifteen minutes is all it takes to install and set up Lionheart FX for image capture and analysis. The filter/LED cubes and objectives are accessed from the front panel, and tool-free connections for injectors, gas control and the environmental cover add to the easy installation. Its small size takes up minimal benchtop space.
Cliente em Destaque
Discovering New Ways to Advance Cell Biology Research with Lionheart FX
16-August-19
Creating an in vitro environment that is highly similar to that found in the body, including the presence of an extracellular matrix (ECM), is a critical aspect of reaping the most relevancy from cell-based research. Often, researchers find that optimizing culture environments is a time-consuming and costly process. The team at denovoMATRIX GmbH in Dresden, Germany, seeks to ease this burden by developing and manufacturing novel, pre-coated cultureware for specific cell types. Dr. Dejan Hušman, CEO of denovoMATRIX, explains that the specialized coatings used on the cultureware are composed of glycosaminoglycans and biomimetic peptides that can be combined to generate entire libraries of extracellular matrices for use in their user-customized myMATRIX and their flagship screenMATRIX products. Dr., Hušman notes that development of the latter was dependent on their use of BioTek’s Lionheart™ FX Automated Microscope.
Certificações
