Recursos - Resumos Visuais
Sub-Micron Spot Quantitation of Centromeres
Related Products: Leitor multimodal com captura de imagens de células Cytation 5, Microscópio automatizado Lionheart FX
July 28, 2017
Related Applications: Immunofluorescence, Phenotypic Assays
The resolving power of an imaging system is the foundation of successful data analysis. This experiment compares a 20x air objective(0.45 NA)to a 100x oil immersion objective(1.42 NA)for the count of sub-micron objects (centromeres) in cell nuclei. Cell nuclei are stained with DAPI and centromeres (CENP-A) areimmunostainedwith Texas Red-labeled antibodies.