Recursos - Resumos Visuais

Non-Alcoholic Steatohepatitis (NASH) Imaging Assay

Related Products: Cytation 1, Leitor multimodal com captura de imagens de células Cytation 5, Microscópio automatizado Lionheart FX, Microscópio Automático Lionheart LX

November 06, 2017

NASH is a condition in which fat builds up in the liver. Understanding the intracellular absorption of free fatty acids and their role in cellular cytotoxicity is critical towards elucidating the causes of NASH. iCell hepatocytes 2.0 from Cellular Dynamics International (CDI) are treated with various concentrations of oleic: palmitic free fatty acid mixture. After incubation (24 hours), cells are fixed and stained with DAPI (nuclei), Texas red phalloidin (cytoskeleton) and BODIPY 493/503 (lipids).