Recursos - Pôsteres Científicos
Investigation of Cell Migration using a High Density Cell Exclusion Assay and Automated Microplate ImagerDownload
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January 21, 2014
Authors: P. Brescia, P. Banks and G. Prescott, BioTek Instruments, Inc., Winooski, Vermont; G. Krishnan, Platypus Technologies, LLC, Madison, Wisconsin
Cell migration, the movement of cells from one location to another, can be the result of complex biological or environmental signaling events. The migration of cells is known to occur during a vast array of both normal biological processes such as the development of an organism or marking pathological events such as cancer metastasis. The need to better understand the process of cell migration has led to the development of improved methods of investigation1 including culturing methods, live cell trackers and detection methods. Improvements in both the investigative methods and throughput technologies will allow for rapid expansion of investigation in the area of cell migration research. This study investigated the use of a novel medium and high density cell exclusion assay for analysis of cell migration. The use of a biocompatible gel (BCG) deposited in a portion of either a 96- or 384-well density microplate was used to exclude cells during seeding. A cell free zone was revealed after dissolution of the BCG following media/cell addition to the wells. After cell attachment, compound addition can be made directly to the wells for investigation. Assay optimization was performed including the pharmacology of a known migration inhibitor, using several cell tracking methods and an automated microplate imager. Data analysis was performed using both image analysis capabilities integrated in the instrument control software, as well as by exporting data for analysis by an open-source image analysis software package.