Apoptosis

Apoptosis

Apoptosis, or programmed cell death, refers to a series of biochemical changes in the cell leading to distinct morphological changes that can be assessed using fluorescent probes or contrast-enhancing microscopy modes such as phase contrast. Examples of fluorescent probes include those that target phosphatidylserine which becomes exposed to extracellular reagents in early stage apoptosis (i.e. pSIVA-IANBD), fluorometric substrates of or antibodies to caspases (i.e. Caspase 3/7) that generate fluorescence with caspase activation, or probes that measure chromatin condensation. Cytation and Lionheart FX both have the ability to measure apoptosis through the use of fluorescent probes or by label-free methods relying on phase contrast microscopy.

 

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